Abstract
A laser light scattering immunoassay was used to measure concentrations of human chorionic gonadotropin (hCG) and human luteinizing hormone (hLH) in urine. In this assay quasi-elastic light scattering spectroscopy is used to detect the inhibiting effect of hCG and hLH on an hCG-hLH co-specific rabbit antibody, which acts to agglutinate hCG coated latex spheres. The results obtained with this assay are quantitatively accurate and the sensitivity of the assay is found to be about 0.015 i.u. (U.S.P. standard)/ml of hCG or hLH experiment. This sensitivitiy permitted the measurement of the pituitary hLH stimulus for ovulation in the menstrual cycle. The agglutination-inhibition mode permits one to assay for a wide variety of molecules including haptenes.
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