Abstract
The dynamics of the enthalpy and volume changes related to the photo-dissociation of oxygen from human and bovine oxyhemoglobin are investigated by nanosecond time-resolved photoacoustic calorimetry (PAC). The values of enthalpy and volume change associated with the above process are Δ H=37.8±3 kcal/mol, Δ V=5.0±1 ml/mol for human HbO 2; and Δ H=35.7±3.5 kcal/mol, Δ V=4.8±1 ml/mol for bovine HbO 2, respectively. A possible explanation for the similar values between both human and bovine oxyhemoglobin is proposed. In addition, the PAC results for human HbO 2 and HbCO are compared and discussed.
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