Abstract
This chapter discusses laser-induced photogeneration of ATP. It presents an experiment to show how ATP hydrolysis in muscle fibers is coupled to muscle contraction. A photochemical method is used in which fibers were incubated with the photo-labile compound P 3 -l-(2-nitro)phenylethyladenosine 5'-triphosphate . ATP was then released rapidly by a pulse of 347 nm irradiation from a frequency-doubled ruby laser. The most straightforward experiment with caged ATP is to measure the kinetics of relaxation of rigor muscle following the release of ATP. The cleavage kinetics of ATP can be followed by using radioactive caged ATP. Within 50 msec of releasing ATP in a muscle fiber, an amount of ADP is formed that is approximately the molar equivalent of the myosin heads in both the absence and presence of calcium ions. This burst of ADP formation is followed by slower steady-state ADP formation.
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