Abstract
Flow microphotometry, a term we have coined to describe the unique flow systems developed over the last few years at the Los Alamos Scientific Laboratory (LASL), allows quantitative precision measurements to be made of the optical properties of individual mammalian cells. Mammalian cells in suspension are made to pass through a special flow chamber where they are lined up, one at a time, for exposure to the blue light from an argon ion laser. As each cell crosses the laser beam, it produces one or more optical pulses of a duration equal to cell transit time across the beam. These pulses are detected, amplified, and analyzed using essentially the techniques of γ-ray spectroscopy.
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