Abstract
In the present work, the two-photon polymerization (2PP) technique was applied to develop precisely defined biodegradable 3D tissue engineering scaffolds. The scaffolds were fabricated via photopolymerization of gelatin modified with methacrylamide moieties. The results indicate that the gelatin derivative (GelMod) preserves its enzymatic degradation capability after photopolymerization. In addition, the developed scaffolds using 2PP support primary adipose-derived stem cell (ASC) adhesion, proliferation and differentiation into the anticipated lineage.
Highlights
Scaffold fabrication is one the most rapidly evolving technological aspects related to tissue engineering
We have previously demonstrated the feasibility to apply two-photon polymerization (2PP) to develop 3D scaffolds starting from conventional photosensitive materials [12] and acrylated PEG [13]
We report on the development of 3D scaffolds using 2PP starting from photosensitive gelatin
Summary
Scaffold fabrication is one the most rapidly evolving technological aspects related to tissue engineering. A large variety of materials including natural, synthetic, and combinations thereof have already been applied as starting materials during the last two decades [1,2,3]. Scaffolds should mimic the natural environment of the targeted tissue. The applied material determines the cell-interactive properties to a large extent, recent findings indicate that the scaffold geometry plays a pivotal role in its ability to mimic the natural cellular environment [4,5,6,7,8]. The final scaffold architecture cannot be fine-tuned using conventional techniques. A series of technologies was elaborated enabling production of scaffolds possessing a well-defined pore shape, size, distribution and interconnectivity starting from a provided design [9,10,11]. The most frequently applied techniques include 3D fiber deposition, fused deposition modeling, selective laser sintering and stereolithography
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
