Abstract
Eleven native transfer RNAs have been prepared so as to maintain their Mg2+ content. Their aqueous Raman spectra show a high, relatively constant amount of order in the ribophosphate backbone, as indicated by the ratio 1.73 +/- 0.05 for I814/I1100 in all samples. Variation in the effectiveness of stacking of guanine and adenine bases is seen, though most of the transfer RNAs studied have a comparable degree of stacking to that found in phenylalanine transfer RNA from yeast, whose tertiary structure has been determined by X-ray crystallography. The spectrum of Escherichia coli 5S RNA indicates that the stacking efficiency of the guanine bases is much higher in 5S RNA than in yeast in phenylalanine transfer RNA, while that of the adenine bases is lower.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
