Laser Doppler flowmetry in assessing the distant stimulating effect by autotransplantation of skin flap on microcirculation

Abstract

Chronic hyperglycemia in diabetes mellitus (DM) is the pathogenetic basis for the occurrence and development of macro- and microvascular disorders, which provoke the development of retinopathy, nephropathy and cause the accelerated development of atherosclerosis with damage to the coronary, cerebral and peripheral vessels. This determines the relevance of developing new methods for the correction of disorders of carbohydrate metabolism, including nonpharmacological ones. Using the method of laser Doppler flowmetry, microcirculation disorders in rats caused by alloxan insulin deficiency were studied. Disruption of microcirculation was manifested by a decrease in perfusion index on the skin of the dorsum of the foot in combination with a decrease in the normalized amplitudes of endothelial and neurogenic oscillations. An animals that have non-pharmacological correction (autotransplantation of the skin flap) of carbohydrate metabolism disorders, an increase in perfusion index relative to the comparison group was observed on day 42 of the experiment, indicating an improvement in microcirculation. An increase in the normalized amplitudes of endothelial and neurogenic oscillations relative to the comparison group was also noted. The data obtained allow us to conclude that the use of laser Doppler fluometry in the study of the functional state of the microvasculature and the diagnosis of vascular complications of diabetes is very promising.