Laser assisted synthesis of inorganic fullerene like MoS2-Au nanohybrid and their cytotoxicity against human monocytic (THP-1) cells

Abstract

Here in, we report the investigation of the immunotoxicity of gold nanoparticles decorated on inorganic fullerene like MoS2 nanostructure (IFMoS2- AuNPs) on the THP-1 immune cell line. The MoS2 nanoparticle with fullerene like nanostructure (IFMoS2) was synthesized by double pulsed laser-assisted chemical vapour deposition (LCVD) method from bulk MoS2 at the temperature of 700 °C. The MoS2 inorganic fullerene-like nanoparticles grown by vapour-solid process (VS). The surface of the IFMoS2 was decorated with gold nanoparticles (AuNPs) to develop the semiconductor biocompatibility interface. The IFMoS2 are typically with diameters 20–50 nm as observed from the electron microscopy analysis. The stability of IFMoS2-AuNPs were examined by dynamic light scattering (DLS) and zeta potential measurements. The decoration of AuNPs on IFMoS2 surface was analyzed by UV–Vis, FTIR, PXRD, FESEM, EDX with elemental mapping and HRTEM measurement. The in vitro cytotoxicity evaluation of the FMoS2-AuNPs was determined on acute monocytic leukemic cells (THP-1) in which cell viability, caspase activity (CASP -3&7/CASP -8/CASP -9) and cell- structural changes were assessed. From FESEM revealed that IFMoS2-AuNPs induced apoptosis in THP-1 cells. Further, THP-1 cell viability markedly decreased at higher IFMoS2-AuNPs concentrations (65–100 μg/ml), indicating its potential as a chemotherapeutic agent in the treatment of human monocytic leukemia.