Abstract
In this work, we applied a constructed multi-photon polymerization system based on diode-pumped solid state femtosecond Yb:KGW laser used as pulsed irradiation light source (300fs, 1030nm, 200kHz) in combination with large area high sample translation velocity (up to 300mm/s) linear motor-driven stages (100×100×50mm3) designed for high resolution and throughput 3D micro/nanofabrication. It enables rapid prototyping out of most polymers up to cm in scale with sub-micrometer spatial resolution. This can be used for production of three-dimensional artificial polymeric scaffolds applied for cell growth and expansion experiments as well as tissue engineering. Biocompatibilities of different acrylate, hybrid organic–inorganic and biodegradable polymeric materials were evaluated experimentally in vitro. Various in size and form scaffolds of biocompatible photopolymers were successfully fabricated having intricate 3D geometry, thus demonstrating the potential of the applied method. Adult rabbit myogenic stem cell proliferation tests show artificial scaffolds to be applicable for biomedical practice. Additionally, a micromolding technique was used for a rapid multiplication of adequate laser manufactured structures.
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