Larvicidal potential of different chromatographic fractions of the n-hexane extract of Artemisia scoparia against the vector mosquito Culex quinquefasciatus

Abstract

The present study aims to explore the larvicidal activity of n-hexane extract isolated fractions from Artemisia scoparia against third instar larvae of Culex quinquefasciatus mosquito. Twelve fractions, F1 to F12 were separated through column chromatography by using different eluent solvents such as hexane, hexane-chloroform, chloroform and chloroform-methanol, in the order of increasing polarity. Larval bioassay was done by using WHO standard guideline. Larvicidal potential of the separated fractions was evaluated at tested concentrations of 30, 60, 90 and 120 ppm. Mortality rate was scored 24 h post exposure. The fractions F3 and F4 were found more active, exhibiting LC50 values of 28.66 and 22.37 ppm and LC90 values of 53.59 and 53.16 ppm respectively. The larvicidal activity of different fractions was compared by using ANOVA analysis.