Abstract

Early instar larvae that lack distinguishing structural characters can be identified quickly and easily with cellulose acetate gel electrophoresis. Examples are given from the genera Ameletus (Ephemeroptera) and Coenagrion, Enallagma, and Lestes (Odonata). We tested 28 or 29 enzymes which yielded up to 29 scorable loci; of these only six or seven were necessary to achieve species separation at three or more loci. The technique facilitates the search for structural characters, confirms species separation based on morphology, and allows the construction of complete life histories and life tables.

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