Abstract
BackgroundThe La-related protein 1 (LARP1) has been found to be a RNA binding protein and was related to spermatogenesis, embryogenesis and cell-cycle progression. The aim of this study was to investigate the prognostic value of LARP1 in hepatocellular carcinoma (HCC).MethodsLARP1 expression was examined in 15 HCC cell lines and 272 clinical specimens using real-time PCR, immunohistochemistry (IHC) and western blot analysis (WB). LARP1 expression was also studied in 6 paired HCC lesions and the adjacent non-cancerous tissue samples. Statistical analyses were applied to derive association between LARP1 expression scores and clinical characters as well as patient survival.ResultsmRNA and protein levels of LARP1 were higher in HCC cell lines and HCC lesions than in normal liver epithelial cells and the paired adjacent noncancerous tissues. LARP1 expression was correlated to survival time, vital status, tumor size and Child-Pugh score. Overall survival analysis showed HCC patients with high LARP1 expression level had lower survival rate (P < 0.01). Importantly, this correlation remained significant in patients with early-stage HCC or with normal serum AFP level.ConclusionsLARP1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.
Highlights
Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed cancers and the third most common cause of cancer mortality worldwide [1]
To determine whether La-related protein 1 (LARP1) upregulation found in liver cancer cell lines was related to clinical biochemical indicators, Western blot analysis was performed with 6 paired hepatocellular carcinoma (HCC) tissues and non-cancerous tissues adjacent to HCC tumors
Association between LARP1 expression and clinical features of liver cancer To determine the clinical significance of LARP1, the correlation between the clinicopathological features of HCC and LARP1 expression was investigated in a retrospective cohort of 272 HCC cases by IHC, including 16 cases of stage I (5.9%), 195 cases of stage II (71.7%) and 61 cases of stage III (22.4%), based on the tumor/lymph node metastasis/distal metastasis (TNM) staging
Summary
Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed cancers and the third most common cause of cancer mortality worldwide [1]. It is of great clinical value to further identify effective early markers for. The progression of HCC is thought to involve the deregulation of genes that are critical to cellular processes such as cell cycle control, cell growth, apoptosis, and cell migration and spreading. Prediction of prognosis plays an essential role in the assessment of HCC patients and optimal therapeutic options. While tremendous effort has been made in identifying effective indicators for the prognostic prediction of these HCC, the availability of clinically applicable biomarkers remains limited. The La-related protein 1 (LARP1) has been found to be a RNA binding protein and was related to spermatogenesis, embryogenesis and cell-cycle progression. The aim of this study was to investigate the prognostic value of LARP1 in hepatocellular carcinoma (HCC)
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