Larinus minutus (Coleoptera: Curculionidae) and Urophora quadrifasciata (Diptera: Tephritidae), Evidence for Interaction and Impact on Spotted Knapweed in Arkansas.

Abstract

Interactions among seed-feeding insects are well noted in the literature, with many of these interactions being asymmetrical and causing a disadvantage for one of the species involved. While often effective, the use of the cumulative stress approach to biological control (where multiple natural enemy species are released) may increase the risk of interaction, which may lead to less effective biological control programs. Spotted knapweed (Centaurea stoebe ssp. micranthos (Gugler) Hayek) is an invasive plant that causes damage in rangelands and pastures. In Arkansas, two biological control agents for spotted knapweed, Larinus minutus Gyllenhal and Urophora quadrifasciata Meigen, are established. Both species attack the capitula of spotted and diffuse knapweeds. There is potential for interactions between these species, and this could affect the efficacy of the biological control program. To evaluate these potential interactions, we collected spotted knapweed capitula weekly from three sites in Arkansas from 2010 to 2012. Capitula were monitored for emergence of L. minutus and U. quadrifasciata, and the effect on seed reduction was analyzed. Over all three years, L. minutus and U. quadrifasciata were present in 35.8% (492) and 22.8% (313) of the capitula, respectively. We determined that the occurrences of L. minutus and U. quadrifasciata were not independent of each other, and that L. minutus is more effective at reducing spotted knapweed seed production than U. quadrifasciata within individual capitula. Seed production in capitula with both natural enemies present was no different from capitula with L. minutus.