Abstract
Carica papaya L. is a fruit tree species that is cultivated worldwide. Its production involves the use of hermaphrodite plants, which produce pear-shaped fruits with abundant pulp, making these fruits of high market value. Female plants are eliminated after the sex of the flowers is determined; this process occurs 5 months after sowing and increases the costs, labor, and time required for production. Here, we describe an effective method of early molecular sex determination from embryogenic callus that allows the propagation of only hermaphrodite emblings of C. papaya ‘Golden’ via somatic embryogenesis. This workflow reduces costs and the time required for C. papaya micropropagation. The present work provides a foundation for the future use of this approach for agronomic purposes. An effective method of early molecular sex determination from embryogenic callus of Carica papaya ‘Golden’ has been developed, allowing the propagation of only hermaphrodite emblings during somatic embryogenesis.
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