Abstract
We present a Computational Miniature Mesoscope that enables 3D fluorescence imaging across an 8-mm field-of-view and 2.5-mm depth-of-field in a single shot, achieving 7-micrometer lateral resolution and better than 200-micrometer axial resolution. The mesoscope has a compact design that integrates a microlens array for imaging and an LED array for excitation on a single platform. Its expanded imaging capability is enabled by computational imaging. We experimentally validate the mesoscopic 3D imaging capability on volumetrically distributed fluorescent beads and fibers. We further quantify the effects of bulk scattering and background fluorescence on phantom experiments.
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