Abstract
A large-scale stirred culture system for the expansion of mouse embryonic stem cells (mESCs) in spinner flasks under serum-free conditions was established using macroporous microcarriers for cell attachment and growth. This type of microcarrier was chosen as it potentially offers more protection to cells against shear stress in the absence of serum compared to microporous ones. In addition, methods to characterize ESCs after large-scale expansion were established. The pluripotency of expanded mESCs was evaluated based on both flow cytometry and alkaline phosphatase staining. Envisaging the application of ESCs as a potential source of neural progenitors, the neural commitment potential of cells after expansion in the spinner flask was also determined by culturing cells in serum-free adherent monolayer conditions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.