Abstract
SummaryA method for large-scale micropropagation from mature plants of Senecio × hybridus is described. Adventitious shoots were initiated on petiole expiants and then excised and subcultured to obtain multiple shoot formation. The macronutrients of Murashige and Skoog (1962) were used. High IAA concentrations (4–8 mgl−1) combined with 1 mgl−1 BAP were most useful for shoot initiation on petioles. The IAA content had to be lowered (0–0.1 mgl−1) when the shoots were subcultured to avoid inhibition of shoot production. The BAP content was varied between 0.1 and 1 mg l−1 to achieve a balance between the size and number of shoots formed. When no BAP or only a little (0.1 mg 1−1) was used in the subculture before transfer to soil, no other treatments were needed to establish root formation. Plants were stored in vitro for 6 months at 10°C in 400 lux without transfer to fresh medium. Some of the plants produced had more than one shoot and all the plants developed and flowered in a normal way.
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