Abstract
A simple and inexpensive device is described which maintains cultures of marine microalgae at predetermined cell densities by the automatic control of harvesting and replenishment of the culture volume with fresh medium. Cultures were maintained at a range of cell densities in 80-litre internally illuminated vessels and the daily cell production yield was evaluated. Maximum yields of 0·8–1·0×10 12 cells day −1 of Isochrysis aff. galbana Green and 0·8–1·2×10 11 cells day −1 of Tetraselmis suecica (Kylin) Butch. were obtained at culture densities of 15–17×10 3 cells μlitre −1 and 19–21×10 2 cells μlitre −1, respectively. Turbidostat cultures of Skeletonema costatum (Grev.) Cleve could not consistently be maintained above 5–7×10 3 cells μlitre −1.
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