Large scale synthesis of dinucleoside monophosphates catalyzed by ribonuclease from Aspergillus clavatus.

Abstract

AbstractA gram scale enzymatic synthesis of eight, dinucleoside monophosphates (ApC, ApU, CpC, CpU, GpC, GpU, UpC, and UpU) is described. The synthesis involves a reaction between the appropriate ribonucleoside‐2′,3′‐cyclie phosphates and cytidine or uridine in the presence of ribonuelease from Aspergillus clavatus at 30°C. The enzyme is removed from the reaction mixture by chromatography on Bio‐Gel P–4, and the dinucleoside monophosphate is further purified by chromatography on a DEAE‐Sephadex A–25, column.A procedure for the large scale preparation of the ribonuclease from Aspergillus clavatus is also described.