Abstract
Rapid and precise identification of microbial species from a mixture of microbes is the key to developing novel treatments for infectious diseases. Such techniques also provide valuable tools to the study of tissue or environmental microbiome with high diversity. Fluorescence in situ hybridization (FISH) technique allows to detect and identify microbes based on the variation in their genome sequence without the need for time-consuming culturing or sequencing. However, this approach is restricted by crosstalk between species due to non-specific binding of FISH probes.
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