Abstract
A human hepatoma-derived growth factor (HDGF) was purified to homogeneity on a large scale by a combination of Biorex-70 cation exchange chromatography and heparin-Sepharose affinity chromatography. HDGF is a cationic 18,500 molecular weight polypeptide, and is active at about 1 ng/ml in promoting cell proliferation.
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