Year-end Sale % OFF 
Abstract
Diagnostic performance of an indirect enzyme-linked immunosorbent assay (I-ELISA) based on a recombinant nucleocapsid protein (rNP) of the Rift Valley fever virus (RVFV) was validated for the detection of the IgG antibody in sheep (n = 3367), goat (n = 2632), and cattle (n = 3819) sera. Validation data sets were dichotomized according to the results of a virus neutralization test in sera obtained from RVF-endemic (Burkina Faso, Democratic Republic of Congo, Mozambique, Senegal, Uganda, and Yemen) and RVF-free countries (France, Poland, and the USA). Cut-off values were defined using the two-graph receiver operating characteristic analysis. Estimates of the diagnostic specificity of the RVFV rNP I-ELISA in animals from RVF-endemic countries ranged from 98.6% (cattle) to 99.5% (sheep) while in those originating from RVF-free countries, they ranged from 97.7% (sheep) to 98.1% (goats). Estimates of the diagnostic sensitivity in ruminants from RVF-endemic countries ranged from 90.7% (cattle) to 100% (goats). The results of this large-scale international validation study demonstrate the high diagnostic accuracy of the RVFV rNP I-ELISA. Standard incubation and inactivation procedures evaluated did not have an adverse effect on the detectable levels of the anti-RVFV IgG in ruminant sera and thus, together with recombinant antigen-based I-ELISA, provide a simple, safe, and robust diagnostic platform that can be automated and carried out outside expensive bio-containment facilities. These advantages are particularly important for less-resourced countries where there is a need to accelerate and improve RVF surveillance and research on epidemiology as well as to advance disease control measures.
Highlights
The geographic expansion of Rift Valley fever virus (RVFV) in the last four decades associated with high health and socio-economic losses is of great concern for veterinary and public health worldwide
Irrespective of the geographic origin, all virus neutralization test (VNT)-negative sera were regarded in this study as reference panels from RVFV non-infected animals, and VNT-positive sera were considered reference panels from animals infected with RVFV (Table 1)
Diagnostic laboratories are tasked with providing analytical results that are based on internationally recognized methods and standards [73,74]
Summary
The geographic expansion of Rift Valley fever virus (RVFV) in the last four decades associated with high health and socio-economic losses is of great concern for veterinary and public health worldwide. Clinical manifestations of Rift Valley fever (RVF) in livestock vary between species and depend largely on the age of the infected animal. RVFV belongs to a group of viral hemorrhagic fever (VHF) agents regarded as a potential bioweapon with high adverse impacts on public health and agriculture [11,12]. RVF may be suspected when there is a sudden outbreak of febrile illness with headache and myalgia in humans, in association with the occurrence of abortions in domestic ruminants and deaths of young animals following heavy rains [3,6,9]. Humans usually become infected following contact with virus-contaminated tissues and body fluids from infected animals, but mosquito bites can transmit the virus [15,16,17]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
