Abstract
BackgroundCell-based regeneration therapies have great potential for application in new areas in clinical medicine, although some obstacles still remain to be overcome for a wide range of clinical applications. One major impediment is the difficulty in large-scale production of cells of interest with reproducibility. Current protocols of cell therapy require a time-consuming and laborious manual process. To solve this problem, we focused on the robotics of an automated and high-throughput cell culture system. Automated robotic cultivation of stem or progenitor cells in clinical trials has not been reported till date. The system AutoCulture® used in this study can automatically replace the culture medium, centrifuge cells, split cells, and take photographs for morphological assessment. We examined the feasibility of this system in a clinical setting.ResultsWe observed similar characteristics by both the culture methods in terms of the growth rate, gene expression profile, cell surface profile by fluorescence-activated cell sorting, surface glycan profile, and genomic DNA stability. These results indicate that AutoCulture® is a feasible method for the cultivation of human cells for regenerative medicine.ConclusionsAn automated cell-processing machine will play important roles in cell therapy and have widespread use from application in multicenter trials to provision of off-the-shelf cell products.
Highlights
Cell-based regeneration therapies have great potential for application in new areas in clinical medicine, some obstacles still remain to be overcome for a wide range of clinical applications
Cellular morphology and growth Calculations based on the net cell number and doubling time obtained in the ALCADIA trial (Additional file 2)
Trypan blue staining revealed no significant difference in cell viability between the culture methods. Both the methods effectively washed out the cells, as indicated by the paucity of adherent cells on discarded culture dishes. These results suggest that manual passage was effectively replicated using AutoCulture®
Summary
Cell-based regeneration therapies have great potential for application in new areas in clinical medicine, some obstacles still remain to be overcome for a wide range of clinical applications. Current protocols of cell therapy require a time-consuming and laborious manual process. To solve this problem, we focused on the robotics of an automated and high-throughput cell culture system. Automated robotic cultivation of stem or progenitor cells in clinical trials has not been reported till date. Myocardium-derived stem/progenitor cells were isolated by several institutes [1,2,3]. These cell populations have the potential to repair the diseased heart, and clinical trials are currently ongoing
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