Abstract
Industrial applications of anchorage-dependent cells require large-scale cell culture with multifunctional monitoring of culture conditions and control of cell behaviour. Here, we introduce a large-scale, integrated, and smart cell-culture platform (LISCCP) that facilitates digital mass culture of anchorage-dependent cells. LISCCP is devised through large-scale integration of ultrathin sensors and stimulator arrays in multiple layers. LISCCP provides real-time, 3D, and multimodal monitoring and localized control of the cultured cells, which thereby allows minimizing operation labour and maximizing cell culture performance. Wireless integration of multiple LISCCPs across multiple incubators further amplifies the culture scale and enables digital monitoring and local control of numerous culture layers, making the large-scale culture more efficient. Thus, LISCCP can transform conventional labour-intensive and high-cost cell cultures into efficient digital mass cell cultures. This platform could be useful for industrial applications of cell cultures such as in vitro toxicity testing of drugs and cosmetics and clinical scale production of cells for cell therapy.
Highlights
Industrial applications of anchorage-dependent cells require large-scale cell culture with multifunctional monitoring of culture conditions and control of cell behaviour
LISCCP (Fig. 1a) consists of four main components: (i) arrays of ultrathin sensors and stimulators, (ii) graphene oxide (GO)coated and 3D-printed polylactic acid (PLA) substrates, (iii) wirelessly controllable data acquisition system composed of home-made printed circuit boards (PCBs) assembled on Arduino DUE boards, and (iv) an in situ culture medium exchange and circulation system
We develop a multifunctional LISCCP for the digital mass culture of anchorage-dependent cells through multi-scale integration of ultrathin sensor and actuator arrays
Summary
Industrial applications of anchorage-dependent cells require large-scale cell culture with multifunctional monitoring of culture conditions and control of cell behaviour. We introduce a large-scale, integrated, and smart cell-culture platform (LISCCP) that facilitates digital mass culture of anchorage-dependent cells. LISCCP can transform conventional labour-intensive and high-cost cell cultures into efficient digital mass cell cultures. This platform could be useful for industrial applications of cell cultures such as in vitro toxicity testing of drugs and cosmetics and clinical scale production of cells for cell therapy. To achieve microscopy-based monitoring of cellular activities such as proliferation and differentiation, cells are periodically taken outside of the incubators This process increases risk of bacterial contamination and abrupt perturbations in culture conditions[3]. We present a large-scale, integrated, and smart cell culture platform (LISCCP) that facilitates the digital mass culture of anchorage-dependent cells. The centralized culture monitoring and control enable dramatic reduction in the culture-operation labor, which can transform labor-intensive high-cost cell culture into efficient digital mass cell culture (e.g., Supplementary Fig. 2)
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