Abstract
The superconducting magnet generates a field and field gradient product that can levitate diamagnetic materials. In this study a specially designed superconducting magnet with a large gradient high magnetic field (LG-HMF), which can provide three apparent gravity levels (μ-g, 1-g, and 2-g), was used to simulate a space-like gravity environment. The effects of LG-HMF on the ultrastructure and function of osteoblast-like cells (MG-63 and MC3T3-E1) and the underlying mechanism were investigated by transmission electromicroscopy (TEM), MTT, and cell western (ICW) assays. Under LG-HMF significant morphologic changes in osteoblast-like cells occurred, including expansion of endoplasmic reticulum and mitochondria, an increased number of lysosomes, distorted microvilli, and aggregates of actin filaments. Compared to controls, cell viability and alkaline phosphatase (ALP) secretion were significantly increased, and collagen I (col I), fibronectin (FN), vinculin, integrin α3, αv, and β1 expression were changed under LG-HMF conditions. In conclusion, LG-HMF affects osteoblast ultrastructure, cell viability, and ALP secretion, and the changes caused by LG-HMF may be related to disrupting col I or FN/αβ1 integrin.
Highlights
The Earth’s natural geomagnetic field is perceived by some animals and used for navigation
The Effects of large gradient high magnetic field (LG-high-magnetic fields (HMFs)) on Osteoblast-like Cell Viability LG-HMF is an extreme man-made environment, it is necessary to determine whether or not cells can survive under such a special environment
The MTT cell viability assay showed that LG-HMF (m-g, 1-g, or 2-g) did not significantly affect the proliferation of MC3T3-E1 and MG-63 cells after 12 h of culturing compared to controls (Fig. 1A and 1D)
Summary
The Earth’s natural geomagnetic field is perceived by some animals and used for navigation. Recent technologic innovations have led to the generation of man-made static magnetic fields up to 10 Tesla (T). Man-made high-magnetic fields (HMFs) are one of the most powerful tools for studying the properties of materials because HMFs couple directly to the electronic charge and magnetic moments of protons, neutrons, and electrons [1]. Scanned patients and machine operators can be exposed to HMFs. Recently, several studies on the biological effects of man-made HMFs at the cellular level have been reported [2,3]. We were interested in how HMFs may affect cells or animals, how HMFs interact with the body, how humans are affected by HMFs, the health risks associated with HMFs, and how we can apply HMFs to biological systems
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
