Abstract
Large-conductance calcium-activated potassium (BK) channels are expressed in the plasma membrane of various cell types. Interestingly, recent studies provide evidence for existence of this channel also in mitochondria. The goal of the present study was to find a candidate for the regulatory component of the large conductance calcium activated potassium channel in neurons. A combined approach of western blot analysis, high-resolution immunofluorescence and immunoelectron microscopy with the use of antibodies directed against four distinct beta subunits demonstrated the presence of the BK channel beta4 subunit in the inner membrane of neuronal mitochondria in rat brain and cultured neurons. Additionally, channel activity was measured with the use of patch-clamp technique.The study was supported by grant from Ministry of Science and Higher Education No P-N/031/2006 and Polish Mitochondrial Network MitoNet.pl.
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