Large- and small-scale preparation of bacteriophage lambda lysate and DNA.

Abstract

BioTechniquesVol. 25, No. 1 BenchmarksOpen AccessLarge- and Small-Scale Preparation of Bacteriophage λ Lysate and DNAMing-Tsan Su, Tyamagondlu V. Venkatesh & Rolf BodmerMing-Tsan Su*Address correspondence to Ming-Tsan Su, Department of Biology, 830 N. University Street, Natural Sciences Building, University of Michigan, Ann Arbor, MI 48109-1048, USA. Internet: E-mail Address: mingtsan@biology.lsa.umich.eduUniversity of Michigan, Ann Arbor, MI, USASearch for more papers by this author, Tyamagondlu V. VenkateshUniversity of Michigan, Ann Arbor, MI, USASearch for more papers by this author & Rolf BodmerUniversity of Michigan, Ann Arbor, MI, USASearch for more papers by this authorPublished Online:15 Aug 2018https://doi.org/10.2144/98251bm08AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByCharacterisation of broad-spectrum phiKZ like jumbo phage and its utilisation in controlling multidrug-resistant Pseudomonas aeruginosa isolatesMicrobial Pathogenesis, Vol. 172Application of novel lytic bacteriophages to control Vibrio parahaemolyticus load in seafood4 January 2022 | Journal of Consumer Protection and Food Safety, Vol. 17, No. 1Identification of in vivo expressed proteins in live attenuated lipopolysaccharide mutant that mediates heterologous protection against Leptospira sppVeterinary Microbiology, Vol. 262Isolation, Characterization, and Comparison of Efficiencies of Bacteriophages to Reduce Planktonic and Biofilm-Associated Staphylococcus aureus13 August 2020 | Journal of Health and Allied Sciences NU, Vol. 10, No. 03Comprehensive Evaluation of the Safety and Efficacy of BAFASAL® Bacteriophage Preparation for the Reduction of Salmonella in the Food Chain10 July 2020 | Viruses, Vol. 12, No. 7Characterization of a bacteriophage with broad host range against strains of Pseudomonas aeruginosa isolated from domestic animals17 June 2019 | BMC Microbiology, Vol. 19, No. 1Complete genome sequences of Aeromonas and Pseudomonas phages as a supportive tool for development of antibacterial treatment in aquaculture8 January 2019 | Virology Journal, Vol. 16, No. 1Nanobiotechnology: Current and Future Perspectives in Combating Microbial Pathogenesis24 January 2020T4-like Escherichia coli phages from the environment carry blaCTX-M24 May 2018 | Letters in Applied Microbiology, Vol. 67, No. 1Nanomedizin auf Phagenbasis: von Sonden zu Therapeutika für eine Präzisionsmedizin12 January 2017 | Angewandte Chemie, Vol. 129, No. 8Phage‐Enabled Nanomedicine: From Probes to Therapeutics in Precision Medicine24 January 2017 | Angewandte Chemie International Edition, Vol. 56, No. 8Isolation and Characterization of the Lytic Cold-Active Bacteriophage MYSP06 from the Mingyong Glacier in China23 October 2015 | Current Microbiology, Vol. 72, No. 2Maximizing yields of virulent phage: The T4/Escherichia coli system as a test caseJournal of Theoretical Biology, Vol. 364Isolation and characterization of broad spectrum bacteriophages lytic to Vibrio harveyi from shrimp farms of Kerala, India14 November 2013 | Letters in Applied Microbiology, Vol. 58, No. 3Chemoprevention utility of silibinin and Cdk4 pathway inhibition in Apc −/+ ice27 March 2013 | BMC Cancer, Vol. 13, No. 1Export of the pneumococcal phage SV1 lysin requires choline-containing teichoic acids and is holin-independent11 December 2012 | Molecular Microbiology, Vol. 87, No. 2Isolation and Partial Characterization of a Virulent Bacteriophage IHQ1 Specific for Aeromonas punctata from Stream Water27 September 2011 | Microbial Ecology, Vol. 63, No. 4The Autolysin LytA Contributes to Efficient Bacteriophage Progeny Release in Streptococcus pneumoniaeJournal of Bacteriology, Vol. 191, No. 17Identification and characterization of bacteriophages specific to the catfish pathogen, Edwardsiella ictaluriJournal of Applied Microbiology, Vol. 105, No. 6Isolation and Characterization of Bacteriophages Infecting the Fish Pathogen Flavobacterium psychrophilumApplied and Environmental Microbiology, Vol. 74, No. 13Prevalence of human pathogenic enteric viruses in bivalve molluscan shellfish and cultured shrimp in south west coast of IndiaInternational Journal of Food Microbiology, Vol. 122, No. 3Molecular characterization of Vibrio harveyi bacteriophages isolated from aquaculture environments along the coast of IndiaEnvironmental Microbiology, Vol. 9, No. 2Antigenic Subclasses of Polytropic Murine Leukemia Virus (MLV) Isolates Reflect Three Distinct Groups of Endogenous Polytropic MLV-Related Sequences in NFS/N MiceJournal of Virology, Vol. 77, No. 19A new bacteriophage, VHML, isolated from a toxin-producing strain of Vibrio harveyi in tropical AustraliaJournal of Applied Microbiology, Vol. 89, No. 4Molecular Evidence for a New Bacteriophage of Borrelia burgdorferiJournal of Bacteriology, Vol. 181, No. 23Mutations in a gene encoding a new oxygen-regulated photoreceptor protein cause dominant retinitis pigmentosaNature Genetics, Vol. 22, No. 3 Vol. 25, No. 1 Follow us on social media for the latest updates Metrics Downloaded 1,460 times History Published online 15 August 2018 Published in print July 1998 Information© 2018 Author(s)PDF download