Abstract

Pancreatic lipase could be immobilized by binding to large agarose beads or by entrapment within a polyacrylamide polymer. Large agarose beads, prepared from native agarose, were preferred for their higher binding capacity and easy intact separation for reuse. The agarose lipase complex is stable for 6 months at 4 °C and pH 7.5. The immobilized lipase preparation maintained 80% of its initial activity even after reusing it eight times. Consequently, the immobilized lipase was recommended for semicontinuous hydrolysis of olive oil and milk fat trigylcerides. The amount of total hydrolysis was 31 ± 1.17% for milk fat compared with 27 ± 1.03 % for olive oil.

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