Abstract
Abstract We introduce a field portable Thin Layer Chromatography (TLC) plate scanner based on digital image processing hardware. The TLC scanner module consists of a Charge Coupled Device (CCD) imaging detector, a Xenon strobe light source, and the necessary hardware to capture and process video images. The instrument is bundled with a laptop computer to form a compact instrument the size of a 6 inch thick briefcase. To automate analysis, software has been developed for (a) subtraction of dark noise, correction of variations due to pixel sensitivity and spatial nonuniformity of source intensity, (b) automated lane finding, (c) comparison to standards for on-plate calibration, and (d) peak finding and integration. Response to analyte concentration is linear with a detection limit of 40 ppb for sulfamethazine spotted on a plate, with correlation coefficient of 0.994.
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