Abstract
ABSTRACT The aim of this work is study the laparoscopic ovum pick-up (LapOPU) technique in spotted paca, describing surgery details, complications and oocyte recovery rate. Nine healthy adult non-pregnant captive females were used, in a total of 39 procedures. When the surgical plane of anaesthesia was achieved, the females were positioned at 20º Trendelenburg. Three 6mm trocars were placed on right and left inguinal and hypogastric regions. Abdomen was inflated with CO2 and the intra-abdominal pressure was stablished in 10mmHg. Follicular punctures were performed moving the ovaries with atraumatic forceps. For punctures, an 18-gauge 3.5 inch long needle attached to a vacuum system with pressure not exceeding 65mmHg was used. Oocytes were recovered into 50mL centrifuge tubes with media composed of PBS supplemented with 10 IU/mL of heparin and kept at 36°C. R Software was used for statistical analysis. Data normality distribution (Shapiro test) and variances homoscedasticity (Bartlett test) were tested and descriptive statistics (mean±SD) was used to present the results. It was only possible to perform LapOPU in 30 of 39 laparoscopies (76.92%). The surgical total time was 37.34 ± 18.53 minutes. The total number of visualized follicles, aspirated follicles, and retrieved oocytes were 502, 415, and 155, respectively. And the same parameters per animal were: 14.34 ± 12.23, 11.86 ± 10.03, and 4.43 ± 4.69 respectively. Oocyte recovery rate was 32.56 ± 27.32%. In conclusion, caudal positioning of portals with slight triangulation allows good viewing of the abdominal cavity and eases the manipulation of the ovaries. Thus this described LapOPU technique is feasible in spotted paca and easy to perform.
Highlights
IntroductionAccording to the International Union for Conservation of Nature, this species is not in danger of extinction (IUCN 2015), but overall its numbers have been much decreased because of hunting and habitat destruction (Queirolo et al, 2008)
Spotted paca is the second largest neotropical rodent (Matamoros 1982)
During the first three procedures, trocars were introduced into the cranial abdominal region, but we found that it was more feasible the positioning of portals with light triangulation directing the instrument in the caudal direction, added to partial handling lateralization of the forelimbs, as described, making the viewing and manipulation of the ovaries easier
Summary
According to the International Union for Conservation of Nature, this species is not in danger of extinction (IUCN 2015), but overall its numbers have been much decreased because of hunting and habitat destruction (Queirolo et al, 2008) It is bred and raised in farms (Guimarães et al, 2008; Hosken and Silveira 2001), the commercial breeding of this species appears as an alternative of conservation, by increasing their population stock, and promoting a reduction in hunting and trafficking pressure, and to fill the growing demand for exotic meats in large urban centers (Lourenço et al 2008). Laparoscopic ovum pick-up (LapOPU) used for in vitro production (IVP) is one of the leading reproductive biotechniques in assisted reproduction, highlighted for being less invasive, provide a quicker recovery and the possibility of being held several times in the same female as already described in some domestic species (Teixeira et al, 2011; Cordeiro et al, 2014; Padilha et al, 2014; Teixeira et al, 2015).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
