Lanthanum Chloride Promoted Proliferation with Enhanced S‐phase Entry and Inhibited Potassium Currents of NIH 3T3 Cells

Abstract

AbstractThe effects of La3+ on proliferation, cell cycles, apoptosis and ion channels were investigated in mouse embryo fibroblast NIH 3T3 cells and its possible mechanisms were explored. Our data showed that La3+ promoted cell proliferation with increased S‐phase entry and inhibited the outward potassium currents in a concentration‐dependent manner in NIH 3T3 cells. La3+ and Ca2+ had synergistic effect on cell proliferation and cell cycles. It showed that Ca2+ was needed for La3+‐promoted cell cycle progression. Using the whole‐cell voltage‐clamp technique, we found that La3+ blocked the outward potassium current in a concentration‐dependent manner in NIH 3T3 cells. Lanthanum ions can increase intracellular Ca2+ concentration through inhibition of potassium currents, which induce a series of physiological changes and improve proliferation of cells. This may be one of the molecular mechanisms of lanthanum ions induced cell proliferation. The present work provides a new perspective for understanding the biological and toxicological effects of lanthanum.