Abstract
It is of considerable importance to develop a fluorescence sensor with high efficiency and good sensitivity in biosensing. In this work, a ratiometric fluorescence sensor was constructed by incorporating Tb3+in situ into a bismuth-based metal-organic framework (Tb3+-NOTT-220) and subsequently utilized for rapid and sensitive detection of serotonin (5-hydroxytryptamine) in serum media. When serotonin was exposed to the sensor, the energy transfer from the ligand to Tb3+ was inhibited upon addition of serotonin, which is accompanied by the increase in the fluorescence intensity of the ligand and reduce the emission of Tb3+. Thus, a ratiometric fluorescence assay can be established by virtue of Tb3+ and ligand as the recognition site for serotonin detection. The developed sensor exhibited good selectivity and sensitivity to serotonin with the limit of detection of 0.57 μM and 1.21 μM in water and serum, respectively. Furthermore, the application of fluorescent hydrogel provides an effective scheme for rapid and portable determination of serotonin. The ratiometric fluorescence sensing system presents a new strategy for simple, reliable and on-site determination of serotonin.
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