Abstract

Two undescribed lanostane triterpenoids, tsugaric acids G ( 1 ) and H ( 2 ), and a known compound, N -(3′ α ,4′ β -dihydroxy-2′ β -(hydroxymethyl)-1′ β -(cyclobutyl) palmitamide ( 3 ) were isolated from the fruiting bodies of Ganoderma tsugae and characterized on the basis of spectroscopic and spectrometric analysis, especially 2D NMR and HRMS. Compounds 1 and 2 were also characterized by an LC-ESI-MS analytical approach and were compared with the fragmentation patterns of reported compounds. Compound 3 significantly exhibited anti-HCV activity. • Two new lanostanoids ( 1 and 2 ) and one known palmitamide ( 3 ) were isolated from Ganoderma tsugae . • The new compounds were discovered by using an LC-ESI-MS analytical approach. • Compound 3 exhibited anti-HCV activity.

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