Abstract
A stable monolayer of the isolated reaction centers (RCs) from a photosynthetic bacterium, Rhodopseudomonas viridis was prepared on a water subphase. The behavior of the monolayer was studied with reference to their surface pressure-area isotherms. The results show that a densely packed monolayer could be obtained by optimizing the initial spreading densities. Langmuir-Blodgett (LB) films which had a dense, close-packed structure could be produced with high initial density of material and high surface pressure. The absorption spectrum of this LB film was similar to that of solubilized RCs. Upon illumination with near infrared light, a sandwich device of LB films exhibited an initial current or voltage transient followed by a steady component. These results indicated that the function of photon/electron conversion of RCs was still retained in the LB films. The LB film fabrication described, giving a steady photocurrent, provides a possible method for the application of membrane proteins in biodevices.
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