Abstract

The studies of model biological membranes consisted of phospholipid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were carried out by means of the Langmuir monolayer technique using subphases containing chitosan (Ch), titanium (IV) oxide (TiO2), hyaluronic acid (HA) or mixture of them. The aim was to determine the effect of individual components of subphase and their respective combinations on behavior of the DPPC membrane. The systems were tested at room temperature (20 °C) and at a natural pH of about 4.8, which was close to the pH of the human skin (4.7–5.6). The surface pressure–area per molecule (π–A) isotherms were obtained. Their analysis showed that all substances studied affected the phospholipid membrane which was revealed in the changes of mean molecular area, compression modulus and pressure of the liquid-expanded/liquid-condensed (LE/LC) phase transition. The results were discussed in terms of the nature and strength of mutual interactions. The more profound effect was found at low surface pressures at which the monolayers occurred in more expanded state. However, at the surface pressure corresponding to that of biological membranes, systems had very similar parameters compared to model DPPC isotherm.

Highlights

  • Chitosan (Ch) is a biopolymer that has recently enjoyed a lot of popularity

  • Compression isotherms are characterized by particular regions that depict the state of the DPPC monolayer: gas (G), decompressed liquid (LE), liquid state (LE)–LC phase transition, and condensed LC

  • Further compression leads to a phase transition from decompressed liquid state (LE) to condensed liquid (LC)

Read more

Summary

Introduction

Chitosan (Ch) is a biopolymer that has recently enjoyed a lot of popularity. This is due to a number of its interesting properties, resulting from its structure. Due to its bioactivity and biodegradability Ch is popular among pharmaceutical, medical and cosmetic concerns. It is used primarily as a support for the supply of drugs and proteins (Moradi et al 2019), biosensors (Zhang et al 2018), nanofibers (scaffolds in tissue engineering) (Rijal et al 2018; Bhowmick et al 2018). In the area of life science, titanium dioxide (­TiO2) is often found, which is generally associated with its role as

Results
Discussion
Conclusion

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.