Abstract
Langmuir and Langmuir-Blodgett films holding a synthetic bioinspired wound healing active compound were used as drug-delivery platforms. Palmitic acid Langmuir monolayers were able to incorporate 2-methyltriclisine, a synthetic Triclisine derivative that showed wound healing activity. The layers proved to be stable and the nanocomposites were transferred to solid substrates. Normal human lung cells (Medical Research Council cell strain 5, MRC-5) were grown over the monomolecular Langmuir-Blodgett films that acted as a drug reservoir and delivery system. The proliferation and migration of the cells were clearly affected by the presence of 2-methyltriclisine in the amphiphilic layers. The methodology is proposed as a simple and reliable model for the study of the effects of bioactive compounds over cellular cultures.
Highlights
As the lipid fractions of biological membranes are essentially formed by different phospholipids, Langmuir [1, 2] and Langmuir Blodgett (LB) [3, 4, 5] films formed by amphiphilic compounds are extensively used as mimetic systems and as models to scrutinize cellular membrane processes
Higuchi et al demonstrated that fibroblast L929 cells cultured on collagen adhered and spread better on synthetic polymeric films prepared by the LB method than on films obtained by the casting method [20]
We reported the effect of the presence of MT in LB layers on the development of human normal lung fibroblast cells (Medical Research Council cell strain 5, MRC-5), cultivated over the monomolecular LB films, that acts as drug reservoir
Summary
As the lipid fractions of biological membranes are essentially formed by different phospholipids, Langmuir [1, 2] and Langmuir Blodgett (LB) [3, 4, 5] films formed by amphiphilic compounds are extensively used as mimetic systems and as models to scrutinize cellular membrane processes This technique has been applied in the study and development of different active compounds, such as antibacterial [6, 7] and antifungal [8] drugs, as well as anti-inflammatory [9], sedative [10], neuroactive drugs [11] and antitumor [12, 13] agents, among others. Hippocampal pyramidal neurons were cultured onto LB films of insulin with different surface packing density, showing that the neuron polarization through the activation of the Insulin-like Growth Factor-1 receptor can be selectively modulated by
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