Abstract
AbstractGinsenoside Rh2 is one of the rare ginsenosides extracted from Panax ginseng C. A. Mey. The anti-allergic activity of ginsenoside Rh2 has been documented in some literature. In this work, an anti-allergic mechanism of ginsenoside Rh2 was investigated by focusing on the differentiation of T cells through Langerhans cells (LCs). Langerhans cell-like dendritic cells (LDCs) were generated in vitro and were used as substitute for LCs.In vivo the mRNA expression for IFN-γ and CXCR3 of T cells was increased after being injected with ginsenoside Rh2-treated LDCs thereby increasing the concentration of IFN-γ in the culture supernatants of CD3+/CD28+ T lymphocytes. However,in vitro, the expression of mRNA for CD40 and CD80 on ginsenoside Rh2-treated LDCs was up-regulated significantly and the endocytic activity of LDCs was down-regulated slightly. These findings indicate that T cells differentiation could be regulated by ginsenoside Rh2 through LDCs in vivo by altering the antigen presenting capacity, maturation and phagocytosis of LDCs.
Highlights
Since ginsenoside Rh2was removed before the injection, it would be appropriate to assume that ginsenoside Rh2 affected the functions of like dendritic cells (LDCs) by acting on LDCs
As the results of RT-PCR shown in Figure2, the mRNA expression of IFN-γ and CXCR3 were increased by ginsenoside Rh2-treated LDCs significantly
We tried to clarify whether the anti-allergic effects of ginsenoside Rh2were realized by regulation of Th1 and Th2 cells differentiation through LDCs
Summary
Dendritic cells (DCs) play a critical role in immunoregulation as antigen presenting cells (APCs). It has been proved that LCs plays a major role in Th1 and Th2 differentiation in a report [11]. Langerhans cell-like dendritic cells (LDCs) are usually generated in vitro and are used as substitute for LCs. Considering that ginsenoside Rh2 has potent antiallergic activity, while its regulation on T cells and LDCs were seldom reported. Considering that ginsenoside Rh2 has potent antiallergic activity, while its regulation on T cells and LDCs were seldom reported This is why, the effects of ginsenoside Rh2on T cells differentiation through LDCs in vivo were investigated in the current work. Langerhans cell-like dendritic cells treated with ginsenoside Rh2 regulate the differentiation
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