Abstract
ObjectiveTo comprehensively analyze the global N6-methyladenosine (m6A) modification pattern in ameloblastoma.Methodsm6A peaks in ameloblastoma and normal oral tissues were detected by MeRIP-seq. Differentially methylated m6A sites within messenger RNAs (mRNAs), long no-coding RNA (lncRNAs) and circular RNA (circRNAs) were identified, followed by functional enrichment analysis. By comprehensively analyzing MeRIP-seq and RNA-seq data, differentially expressed mRNAs, lncRNAs and circRNAs containing differentially methylated sites were identified. RNA binding proteins (RBPs) were then identified for differentially methylated m6A sites.ResultsIn total, 3,673 differentially methylated m6A sites within coding genes were detected, of which 16.2% (704/3,673) were significantly upmethylated sites in ameloblastoma compared to normal oral tissues. Furthermore, 4,975 differentially methylated m6A sites within lncRNAs were identified, of which 29.4% (1,465/4,975) were upmethylated sites in ameloblastoma. We also found 364 differentially methylated m6A sites within circRNAs, of which 22.5% (82/364) were upmethylated sites in ameloblastoma. Differentially methylated m6A was most often harbored in the CDS (54.10%), followed by 5’UTR (21.71%). Functional enrichment analysis revealed that m6A modification could be involved in the development of ameloblastoma by organism developmental processes. A total of 158 RBPs within differentially methylated m6A sites were identified, which were significantly involved in mRNA metabolic process, mRNA processing, RNA processing, RNA splicing and RNA transport.ConclusionOur findings for the first time provide m6A landscape of human ameloblastoma, which expand the understanding of m6A modifications and uncover regulation of lncRNAs and circRNAs through m6A modification in ameloblastoma.
Highlights
Ameloblastoma is the most common epithelial odontogenic neoplasm globally, accounting for approximately 36% of all odontogenic tumors [1]
There were 6,870 nonoverlapping m6A peaks within messenger RNA (mRNA) (Figure 1A), 4,558 nonoverlapping m6A peaks within long no-coding RNA (lncRNA) (Figure 1B), and 1,091 nonoverlapping m6A peaks within circular RNA (circRNA) (Figure 1B) in ameloblastoma tissues compared to adjacent normal oral tissues
Most of m6A-motified mRNAs (Figure 1G), lncRNAs (Figure 1H) and circRNAs (Figure 1I) contained only one m6A peak, while a small number of them contained two or more, which was consistent with previous studies such as clear cell renal cell carcinoma [23]
Summary
To comprehensively analyze the global N6-methyladenosine (m6A) modification pattern in ameloblastoma. Methods: m6A peaks in ameloblastoma and normal oral tissues were detected by MeRIP-seq. Methylated m6A sites within messenger RNAs (mRNAs), long nocoding RNA (lncRNAs) and circular RNA (circRNAs) were identified, followed by functional enrichment analysis. By comprehensively analyzing MeRIP-seq and RNA-seq data, differentially expressed mRNAs, lncRNAs and circRNAs containing differentially methylated sites were identified. RNA binding proteins (RBPs) were identified for differentially methylated m6A sites
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
