LAMP3 (CD208) Expression in Squamous Cell Carcinoma and Epithelial Dysplasia of the Oral Cavity and Clinicopathological Characteristics of Unfavorable Prognosis.

Mehdi Shahabinejad,Zeynab Asadi,Farnaz Mohajertehran,Reza Zare

doi:10.52547/rbmb.9.4.373

Mehdi Shahabinejad, Zeynab Asadi + Show 2 more

Open Access

https://doi.org/10.52547/rbmb.9.4.373

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Abstract

This study aimed to evaluate LAMP3 (CD208) gene expression in oral squamous cell carcinoma (OSCC) and dysplastic oral epithelium by quantitative real-time polymerase chain reaction (qPCR) and compare LAMP3 expression in different disease grades and stages. In this study, 60 OSCC and dysplastic oral epithelium samples were obtained from the Mashhad University of Medical Sciences together with their demographic and clinicopathological documents. LAMP3 expression was measured by qPCR. LAMP3 expression was significantly greater in OSCC than in dysplasia samples (P=0.001), in grade III OSCC than in grades I and II, and also greater in advanced than in early OSCC disease stage (P=0.001). The significantly greater LAMP3 expression in OSCC than in dysplastic epithelium indicates a role for LAMP3 in carcinogenesis in oral mucosa. Our results suggest LAMP3 may be useful as an anticancer target and/or to predict disease pathogenesis in OSCC patient's cells.

Highlights

Oral squamous cell carcinoma (OSCC) is a major subcategory of head and neck squamous cell carcinomas and accounts for more than 90% of oral cavity cancers [1]
LAMP3 expression LAMP3 expression was significantly greater in oral squamous cell carcinoma (OSCC) than in dysplasia patients. (P=0.001; Table 3)
LAMP3 expression was significantly greater in dysplasia tissue specimens from patients under 60 years of age than in those 60 years and older (P=0.01; Table 4); LAMP3 expression was not significantly different in OSCC samples from the two age groups (P=0.09)

Summary

Introduction

Oral squamous cell carcinoma (OSCC) is a major subcategory of head and neck squamous cell carcinomas and accounts for more than 90% of oral cavity cancers [1]. Increased LAMP3 expression is associated with unfavorable prognosis in many cancers including esophageal squamous cell carcinoma (ESCC) [7], gastrointestinal stromal tumor (GIST) [8], breast cancers [9, 10], cervical cancer [11], and head and neck squamous cell carcinomas [6] This marker is associated with induction and promotion of tumor migration and invasion [12, 13]. Investigations of LAMP3 expression and its association with OSCC patients’ clinical features and pathological characteristics are lacking; we aimed to analyze LAMP3 expression in OSCC tissue samples and dysplastic epithelium by quantitative polymerase chain reaction (qPCR). This study aimed to evaluate LAMP3 (CD208) gene expression in oral squamous cell carcinoma (OSCC) and dysplastic oral epithelium by quantitative real-time polymerase chain reaction (qPCR) and compare LAMP3 expression in different disease grades and stages. Our results suggest LAMP3 may be useful as an anticancer target and/or to predict disease pathogenesis in OSCC patient’s cells

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