Abstract
Purpose: To identify laminitis‐specific plasma microRNAs in a horse Method and materials: In order to induce laminitis experimentally, oligofructose was administered to a horse through nasogastric tube. Peripheral blood was collected from the jugular vein at the time of pre/post induction of laminitis. After euthanasia, various tissues including laminae were collected from the horse. Total RNA was extracted and subjected to the reverse transcription to cDNA. The cDNA products of the small RNA fragments ranging from 40bp to 60bp were sequenced directly using Illumina HiSeq 2000 sequencer.Results: A total of 288 miRNAs were identified in laminar tissues and 262 miRNAs were identified in the plasma of horse with laminitis. Fifteen miRNAs were identified as laminitis‐specific plasma miRNAs, based on the following criteria: laminar tissue‐specific expression compared to other tissues and significant increase of the plasma miRNA level after the induction of laminitis.Conclusions: The present study revealed a subset of plasma miRNAs specific to horse laminitis, suggesting the potential value of the miRNAs as a biomarker for the diagnosis of equine laminitis.Grant Funding Source: Supported by Bio‐industry Technology Development Program of iPET, Ministry of Agriculture, Korea
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