Laminar and spatial localization of the islands of Calleja in mice

Abstract

The islands of Calleja (IC) are dense clusters of cells localized within the ventral striatum. The IC have been described as variable in both number and localization from animal-to-animal, however, a quantitative investigation of this variability is unavailable. Further, it is presently unknown whether the IC occupy select areas of the olfactory tubercle (OT), the ventral striatum structure which possesses the IC in mice. To address these questions, we examined the IC of adult C57bl/6 mice. As previously noted, we found substantial inter-hemispheric and inter-mouse variations in the total number of IC. While the IC were observed in all three cell layers of the OT, the bulk of IC occupied layer iii. The span of the IC along the anterior–posterior and medial–lateral axes of the OT was variant. Further, localizations of the IC within the OT also differed across animals. Notably, the probability of observing an IC in the medial OT was greater than that of observing one in the lateral. These data provide a fundamental characterization of both differences and similarities regarding the IC in mice and will be informative for future in vivo studies seeking to perturb and possibly record from the IC. Further, we predict that inter-animal diversity in the IC may be a mechanism for inter-animal differences in behavior, especially reward-related and motivational behaviors.