LafK contributes the regulation of swarming motility of Pseudomonas plecoglossicida and bacterial-host interaction

Abstract

Flagella-mediated swarming motility plays a crucial role in facilitating the rapid colonization and dissemination of bacterial within the host. The swarming motility of Pseudomonas plecoglossicida is intricately associated with its lateral flagella, and notably, the lateral flagella system of P. plecoglossicida encompasses a transcriptional regulator known as LafK. However, the regulatory role of LafK and its impact on bacteria-host interactions remain to be elucidated. In this study, we systematically investigated the regulatory role of LafK by constructing lafK deletion strain on the biological characteristics, virulence, and pathogenic process of P. plecoglossicida, as well as its impact on the host immune response. Our findings demonstrated that the deletion of lafK led to a significant down-regulation in the expression of type III secretion system-associated genes within the lateral flagella of P. plecoglossicida, consequently impairing bacterial swarming motility, biofilm formation, adhesion, and chemotaxis ability. Furthermore, in vitro infection experiments demonstrated that the deletion of lafK resulted in a diminished pathogenicity of P. plecoglossicida through down-regulation of flagella-related genes, thereby triggering an expedited immune response for bacterial clearance, and subsequently leading to reduced bacterial load within the host and attenuated tissue damage during infection. In summary, this study presents a novel theoretical framework for elucidating the regulatory mechanism of virulence in P. plecoglossicida.