Lactotransferrin is the major estrogen inducible protein of mouse uterine secretions.

B.T Pentecost,C.T Teng

doi:10.1016/s0021-9258(18)61088-9

Abstract

Antibody to an estrogen inducible mouse uterine protein (Teng, C. T., Walker, M. P., Bhattacharyya, S. N., Klapper, D. G., DiAugustine, R. P., and McLachlan, J. A. (1986) Biochem. J. 240, 413-422) has been used to isolate cDNA to the messenger RNA. Analysis of the deduced primary structure and additional biochemical characterization indicates that the protein is lactotransferrin. An increase in the level of lactotransferrin mRNA of at least 300-fold can be induced in the mouse uterus by estrogen. In contrast, the mRNA is virtually undetectable in rat uterine tissue following estrogen administration. The estrogenic stimulation in mouse uterus contrasts with the known prolactin dependence in mammary gland.

Highlights

From the Developmental Endocrinologyand PharmacologySection, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina27709
Analysis of the deduced primary structure and additional biochemical characterization indicates that the protein is lactotransferrin
In this report we detail the isolation of a cDNA to the message for the inducible, estrogen-dependentproteinand demonstrateitsidentitytolactotransferrin which is secreted by the lactating mammarygland [8]

Summary

EXPERIMENTALPROCEDURES AND RESULTS’

Phage from the mouse uterine cDNA libraryin Xgtll were immunochemically screened using antibody to the estrogenin mammary gland. Gen inducible protein was confirmed by identification of de- Genbank"andNational Biomedical Research Foundation duced peptide sequence (underlined in Fig. 2) corresponding data bases and significanhtomology to the transferrinfamily to that of the N-terminal sequence of a CNBr fragment [7] detected.Literature surveys could not locatepublished sein all positions except the first. Careful evaluation of both quence for mouse transferrin, sucha sequence was DNA and peptide sequencing data hafsailed to eliminate the isolated by immunoscreening of a mouse liver Xgtll library single difference.

11. L T F HLTF HTF MTF

Findings

DISCUSSION