Lactosporin loaded electrospun nanofibrous membrane: Novel antibacterial and wound dressing patch

Abstract

IntroductionAny disruption in the cohesion of skin tissue is called a skin ulcer. If the skin lesion is a superficial lesion, it heals easily. But some wounds, such as diabetic wounds, bedsores, and burns, are difficult to heal and are often associated with infection. Therefore, one of the new methods used to treat such wounds is skin patch. MethodIn this study, partly purified lactosporin (LP), as novel postbiotic, was extracted from Bacillus coagulans, and its antibacterial properties against six bacterial species, namely Escherichia coli, Micrococcus Luteus, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermis, and Klebsiella pneumonia were investigated. An electrospun nanofibrous membrane (NFM) containing LP was electrospun based on the three polymers including hyaluronic acid (HA), chitosan, and polyvinyl alcohol (PVA) and its potential for biomedical applications was analized. The morphological characteristics, hydrophilic properties, cytotoxicity, and wound healing potential of LP-loaded NFMs were investigated. ResultsIt was revealed that extracted LP includes supperior antibacterial properties, which is beneficial for developing wound dressing patches. The results showed fabricated composite NFMs are bead-free and uniform. The average diameter of uncross-linked and cross-linked nanofibers are 281 ± 10 nm and 359 ± 51 nm, respectively. These NFMs are hydrophilic in both cross-linked and uncross-linked forms and the contact angles of them were 39 and 13°, respectively. These NFMs were also tested for cytotoxicity by the MTT assay and were found to be non-toxic. ConclusionThese results indicate that HA/CS/PVA/LP NFM could act as an efficient antibacterial and multifunctional wound dressing patch.