Abstract
The presence of nonspecific UDP-galactose hydrolases in mammary tissue homogenates interferes with the radioactive assays of the A and B components of lactose synthetase. Uridine triphosphate was added to assay incubation mixtures and was found to effectively inhibit the hydrolase activity with a minimal influence on the specific A and B protein activities. This effect was present over a wide range of mammary tissue homogenate protein concentrations and incubation times. It is suggested that this modification will improve the specificity of existing radioactive assays of lactose synthetase.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Paper version not known
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
