Abstract
Semicontinuous and continuous hydrolysis of lactose in aqueous two-phase systems (polyethylene glycol 20000/dextran 40) with whole-cellβ-galactosidase ofK. marxianus were studied. Both phase polymers had no effect onβ-galactosidase activity confined in cells. Good operational stability of the biocatalyst during 55 cycles of semicontinuous process was observed without appreciable decrease in product concentration. Continuous hydrolysis of lactose was performed in the stirred bioreactor, connected with the phase separator. The satisfactory degree of hydrolysis (between 82–88%) and volumetric productivity (21.6 g/l/h) were reached during 72 hours of continuous hydrolysis of 5% (w/w) lactose.
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