Abstract
Previous observations1 indicated that pituitary glands from lac-tating guinea pigs contained more lactogen than did glands from sexually mature, non-pregnant, and non-lactating guinea pigs. The intent of this assay work was to compare the lactogen content of pituitary glands from guinea pigs sacrificed in estrum, diestrum, early pregnancy, late pregnancy, and lactation.Series I. Animals were paired on the basis of body weight, the average body weight of each group being approximately the same. Six guinea pigs were sacrificed in each of the previously mentioned stages.Series II. Six animals were sacrificed in early pregnancy, late pregnancy, and lactation. However, the average body weights of these groups varied considerably. Lactating animals in both series were sacrificed on the 11th day of the lactation period, the young having been removed 15 hours previously.The pituitary glands were removed, weighed, and assayed for their lactogen content by injecting the suspended tissue intradermally over the crop ...
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