Abstract
In order to compare infection route between different virulence streptococcus strains, we have chosen Lactococcus garvieae originated from rainbow trout (Oncorhynchus mykiss) as an infection model. After in vivo virulence test, we found high virulence strain R0726 and low virulence strain RT-2. Surface antigen analyzed by western blotting and cannot find significant difference between two strains. After infection L. garvieae into rainbow trout, both strains could be isolated from spleen 2h post-infection. We cannot isolate RT-2 after 18th day post-infection, but after the 24th day post-infection we still can isolate R0726. Phagocytosed RT-2 were killed while R0726 significantly surviving within phagosomes of Macrophages.
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