Abstract
ABSTRACT The objective of this study was to isolate, identify, preserve and determine the quantitative level of the Lactobacillus strains from the gut content of 45-day-old chickens broilers; to test the viability of these strains preserved at 4 oC and room temperature (20 ± 2 oC). Lactobacillus strains were isolated, phenotypically identified and preserved from the gut content of 17 chickens broilers. Identification was performed by morphological, cultural and biochemical characters examination, using apiwebTM and ABIS online software. The quantitative level of Lactobacillus strains in intestinal content (105 - 109 CFU/g) and the viability of strains preserved at 4 oC and at room temperature (from 8 days to 9 months) was also determined. Twenty-three strains of L. acidophilus, L. brevis, L. plantarum, L. fermentum and L. salivarius from the gut content of chickens broilers were isolated, phenotypically identified, and preserved. Of these, L. plantarum, L. fermentum and L. acidophilus biotype 1 strains were technologically and ecologically suitable to continue the testing of probiotic traits.
Highlights
Recent research on the structure of the normal intestinal microbiota of chickens revealed the presence of Lactobacillus spp. (Lu et al, 2003; Wei et al, 2013; Waite & Tailor, 2014; Duar et al, 2017), known for its beneficial effects on the host’s health
Twenty-three strains of the genus Lactobacillus (L. acidophilus biotype 1 IBNA 64, L. acidophilus biotype 3 IBNA 49, 51, 53, 55, 63, 65, L. plantarum biotype 1 IBNA 45, 46, 48, 61, L. salivarius IBNA 47, 52, 54, 59, 60, 62, 67, 68, L. brevis biotype 2 IBNA 50, and L. fermentum biotype 1 IBNA 56, 57, 69) were isolated, identified and preserved from the intestinal content, from seventeen 45 d-old chickens
L. plantarum, L. fermentum biotype 1, L. brevis biotype 2 and L. acidophilus biotype 1 isolates resisted for the longest period of time, 66 days to 9 months at 4 oC and 45 days to 3 months at room temperature
Summary
Recent research on the structure of the normal intestinal microbiota of chickens revealed the presence of Lactobacillus spp. (Lu et al, 2003; Wei et al, 2013; Waite & Tailor, 2014; Duar et al, 2017), known for its beneficial effects on the host’s health. Zou et al (2018) showed that Lactobacillus induce a polarizing effect on the chicken cecal microbiome, suggesting a major influential role of this genus in local microbiome, with negative (with Ruminococcaceae, Lachnospiraceae) or positive (with other lactobacilli, Bacteroides, Clostridiales and Christensenellaceae) correlations. After the 14th day of age, cecum and small intestine of broilers chicks develop various communities (Pedroso & Lee, 2015) but, from day 21 to 42 of age, Lactobacillus became the most abundant organism in the small intestine. Of this genus, L. salivarius, L. johnsoni, L. reuteri, L. oris and L. crispatus were detected (Nakphaichit et al, 2011). This diversity raises the issue of selecting the best strain for developing bacterial-based feed additives in poultry nutrition
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