Abstract
The aim of our study was to evaluate the anti-obesity effects of Lactobacillus sakei (L. sakei) ADM14 administration in a high-fat diet-induced obese mouse model and the resulting changes in the intestinal microbiota. Prior to in vivo testing, L. sakei ADM14 was shown to inhibit adipogenesis through in vitro test and genetic analysis. Subsequently, mice were orally administered 0.85% saline supplemented or not with L. sakei ADM14 to high-fat diet group and normal diet group daily. The results showed that administration of L. sakei ADM14 reduced weight gain, epididymal fat expansion, and total blood cholesterol and glucose levels, and significantly decreased expression of lipid-related genes in the epididymal fat pad. Administration of L. sakei ADM14 showed improvement in terms of energy harvesting while restoring the Firmicutes to Bacteroidetes ratio and also increased the relative abundance of specific microbial taxa such as Bacteroides faecichinchillae and Alistipes, which are abundant in non-obese people. L. sakei ADM14 affected the modulation of gut microbiota, altered the strain profile of short-chain fatty acid production in the cecum and enhanced the stimulation of butyrate production. Overall, L. sakei ADM14 showed potential as a therapeutic probiotic supplement for metabolic disorders, confirming the positive changes of in vivo indicators and controlling gut microbiota in a high-fat diet-induced obese mouse model.
Highlights
Obesity, which is widespread worldwide, is a serious threat to public health, as a factor associated with many metabolic diseases [1]
Pre-adipocyte 3T3-L1 cells were treated with L. sakei ADM14 extract at various concentrations
The cytotoxicity of L. sakei ADM14 extract against 3T3-L1 cells was tested by MTS assay, and no significant difference was observed at any concentration (Figure 1C)
Summary
Obesity, which is widespread worldwide, is a serious threat to public health, as a factor associated with many metabolic diseases [1]. Recent studies have shown that obesity is associated strongly with the bacterial gut microbiome, which is manifested in specific bacterial compositions and functional alterations [4]. These changes can lead to dysbiosis, which occurs in obesity. Previous studies have shown that weight gain and metabolic disorders occur when gut microbiota of obese mice are inoculated into normal or germ-free mice [5]. Specific gut microbiota are associated with obesity, and the ratio of representative dominant strains Firmicutes and Bacteroidetes is a main factor. The modulation of gut microbiota composition represents an attractive potential treatment for metabolic disorders [8]
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